Ion Beam Analysis of Metals in Proteins
Geoffrey W. Grime

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R.J. Ragotte et al. Nat Commun 13, 933 (2022).

DOI: https://doi.org/10.1038/s41467-022-28601-4

Examples
Malaria vaccines: do our antibodies help each other?

Need to understand mechanism of synergy of antibodies against malaria to improve vaccine design. Calcium appears vital for maximum response.

          ☐  CyRPA is a protein of the malaria parasite.

          ☐  A panel of antibodies that bind to CyRPA and strongly inhibit                   parasite growth in vitro (Cy.00x) were tested and their 3-D                      structures found.

           ☐  PIXE used to identify and quantify metals and confirmed that                  calcium was vital to the binding.

The presence of iron in PhoX raises the possibility that microbial phosphate acquisition is limited by iron bio-availability.