Multimodal Imaging: Elements & Molecules in Biological Samples
Catia Costa & Melanie J. Bailey

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Costa, C et al. Metabolites 2023, 13, 262.
DOI: https://doi.org/10.3390/metabo13020262

Desorption electrospray ionisation (DESI) and particle induced X-ray emission (PIXE)

☐  Using a spray solvent consisting of 50:50 (%v/v) methanol:ethanol           enables sequential molecular (DESI) elemental (PIXE) mapping out         on the same tissue section.

☐  This novel spray solvent mixture does not cause measurable loss or         delocalisation of elemental signatures.

☐  This is desirable to allow accurate correlation of elemental and               molecular features since regions of interest are not always                       accurately reproduced in sequential sections.  In this work, the new         solvent system produced similar, if not better, lipid coverage and             sensitivity in positive ion mode when compared to the conventional       methanol:water solvent.

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