Ion Beam Analysis of Metals in Proteins
Geoffrey W. Grime

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Shee Chien Yong et al. Science 345,1170 1173 (2014).

DOI: 10.1126/science.1254237

Examples
P. fluorescens PhoX alkaline phosphatase

Uptake of phosphate by micro-organisms is mediated by phosphatases such as PhoX

  • PhoX contains an active-site cofactor with 5 metal binding sites
  • PIXE indicated 3 Ca ions and 2 Fe ions.

  • No other candidate metals (Mg, Mn, Co, Ni, Zn) were detected.

The presence of iron in PhoX raises the possibility that microbial phosphate acquisition is limited by iron bio-availability.