Multimodal Imaging: Elements & Molecules in Biological Samples
Catia Costa & Melanie J. Bailey

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Catia Costa, et al. Analytical Chemistry 2025 97 (30), 16573-16582

DOI: 10.1021/acs.analchem.5c02890

Secondary ion mass spectrometry (SIMS) and particle induced X-ray emission (PIXE)

☐  Sequential molecular and elemental mapping using water cluster SIMS and PIXE.

☐  Developed a workflow that can be applied to biological
     tissues/cells for
multimodal elemental and molecular imaging at            the micron scale using keV and MeV ion beams on the same sample
☐  This work opens up the exciting prospect of colocalizing metals or       other elements with metabolites and lipids in biological systems             below the single cell level, and future work will need to explore             how  to deal with the difference in information depths

Multimodal ion beam imaging facility EPSRC grant EP/Y015134/1

The proposed new system will incorporate a design where the quality of the mass spectrometry is independent of the nature of the primary ion beam used, and will capitalise on the optimised sample handling, ion transmission and data handling of the commercialised keV SIMS system. The addition of the MeV ion beam into the system is unprecedented internationally and will be provided by the existing tandem accelerator at the SIBC.

System will include:
☐  Focussed MeV ion beam for conventional IBA and MeV-SIMS
☐  Water cluster (keV) primary ion beam
☐  C60 (kV) primary ion beam
☐  TOF analyser for keV and MeV-SIMS experiments
☐  X-ray and particle detectors for conventional IBA

When installed and commissioned (early 2026), the system will allow:
☐  Elemental imaging (IBA)
☐  Depth profiling (IBA)
☐  3D imaging of small and large molecules (keV and MeV-SIMS)
☐  Multimodal imaging of elemental and molecular species (SIMS and IBA)
☐  Multimodal depth profiling (SIMS and IBA)